APPLICATION OF PEROXIDASE-LABELED ANTIBODY TO IMMUNOCYTOCHEMISTRY

Binding of specific peroxidase-labeled antibody to placental-type phosphatase on tumor-derived membrane fragments.

Monospecific antiserum to human placental alkaline phosphatase was purified by immunoabsorption and labeled with horseradish peroxidase. The binding of this labeled antibody to membrane fragments prepared from placental and tumor tissue was measured using agarose gel filtration to separate bound antibody. The antibody bound only to membrane fragments which contained placental phosphatase, and t...

Overview of antibody use in immunocytochemistry.

Use of horseradish peroxidase-labeled antibody for light and electron microscope localization of reovirus antigen.

Horseradish peroxidase-labeled antibody was used for light and electron microscopic localization of reovirus antigen in tissue culture. Reaction product in infected cells was easily detected in the cytoplasm, and the procedure was as sensitive as the fluorescent-antibody technique. At the electron microscopic level, infected and enzyme-labeled antibody-treated cells showed accumulations of reac...

Local action of intrahypophyseal implants of estrogen as revealed by staining with peroxidase-labeled antibody.

The direct action of 17 p-estradiol and cholesterol on cells of the pituitary pars distalis, as revealed by staining with peroxidase-labeled antibody, was studied in female rats. Pellets of pure cholesterol and of estradiol mixed with cholesterol were implanted into the left lobe of the pars distalis 14-32 days after ovariectomy and left in place for 7-16 days. Rabbit antisera to rat prolactin,...

Immunocytochemical localization of simian virus 40 T antigen with peroxidase-labeled antibody fragments.

T antigen was localized in simian virus 40 lytically infected and transformed cells by Fab' antibody conjugates at the ultrastructural level. This virus-specific protein appeared in the cytoplasm of permissive cells as early as 3 h after infection. At later time intervals, the T antigen was localized in both the cytoplasm and nucleus and finally (24 h) in the nucleus. These results suggest a sy...